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KMID : 0368420010440040179
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Journal of Plant Biology
2001 Volume.44 No. 4 p.179 ~ p.184
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Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer)
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Yu Kee-Won
Gao Wen Yuan
Hahn Eun-Joo
Paek Kee-Yoeup
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Abstract
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We investigated the effects on ginseng adventitious root growth and ginsenoside production when macro-element concentrations and nitrogen source were manipulated in the culture media. Biomass growth was greatest in the medium supplemented with 0.5-strength NH4PO3, whereas ginsenoside accumulation was highest (9.90 mg g-1 DW) in the absence of NH4PO3. At levels of 1.0-strength KNO3, root growth was maximum, but a 2.0 strength of KNO3 led to the greatest ginsenoside content (9.85 mg g-l). High concentrations of MgSO4 were most favorable for both root growth and ginsenoside accumulation (up to 8.89 mg g-1 DW). Root growth and ginsenoside content also increased in proportion to the concentration of CaCI2 in the medium, with the greatest accumulation of ginsenoside (8.91 mg g-1 DW) occurring at a 2.0 strength. The NH4/NO3 -- ratio also influenced adventitious root growth and ginsenoside production; both parameters were greater when the NO3 - concentration was higher than that of NH4 +. Maximum root growth was achieved at an NH4 +/NO3 - ratio of 7.19/18.50, while ginsenoside production was greatest (83.37 mg L-1) when NO3 - was used as the sole N source.
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KEYWORD
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bioreactor, macro element, NH4 +/NO3 - ratio, nitrogen source
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